English: Predicted epitopes vs. the actual epitopes of HEL. (A) The 3-D structure of HEL (CPK representation) together with three Abs (ribbon representation). PDB IDs 1JHL, 3D9A, and 1MLC were superimposed according to HEL structure. Epitope residues are colored blue, green, and red according to the corresponding Ab. Residues that are common to two epitopes are colored orange. (B) The structure of HEL colored according to the same three epitopes as in (A), presented in a different orientation. (C) The structure of HEL colored according to the epitopes predicted by Discotope (light blue), ellipro (purple), and seppa (pink). Note, not all predicted residues of Discotope and ellipro are observable in the presented orientation.